WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol (1987). Herwadkar A, Banga AK. Chem. Int J Pharm Investig. Colloid and Polymer Science, 286, 1223-1231. Bahary, W.S. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Dextran Influence of molecular weight on Webwhittier union high school district superintendent. Macromolecules, 29, 2265-2268. Google Scholar. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Biophysical Journal, 27, 177-181. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. 2006;3(3):37385. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Chemical penetration enhancers for transdermal drug delivery systems. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Journal of Physical Chemistry. Sci. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. International Journal of Pharmaceutics, 277, 99-104. Carbohydrate Research, 340, 811-826. Nat Biotechnol. Hindered diffusion of dextran and ficoll in microporous membranes. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Learn More. J. Braz. The micropore formed at the SC through to the epidermis, enabling drug delivery. ; Talsma, H.; Hennink, W.E. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. GYA primarily conducted research. Determination of intrinsic viscosities of macromolecules and nanoparticles. Before Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t Viswanath, D.S. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. 2. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Pavlov, G.M. However, in vitro investigations are very few. Walkenhorst, S.; Olivier, R. (2002). Int. Guner, Ali (1999). Kasaai, Mohammad R. (2007). dextran 2009;301(8):60913. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Epub 2012 May 28. Alternatives to animal testing: a review. Carbohydrate Polymers 88 373-381. Recent Pat Drug Deliv Formul. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). Webdextran hydrodynamic radius. ; Ross-Murphy, S.B. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. 2011;16(2):518. ACS Nano. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. De Belder, A. N. (2001). Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Journal of Applied Polymer Science, 13, 2031-2032. The Transdermal diffusion cell system was protected from light. ; Patel, T.R. Bethesda, MD 20894, Web Policies The solution was measured using an Anton Paar DMA35N densitometer. Citation-(RIS Epub 2012 Oct 18. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Arch Dermatol Res. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Harding, Stephen E. (2005). Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. Ed., 9(2), 85-90. 2010;4(1):117. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. (2013). Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). ; Bos, G.W. Microporation applications for enhancing drug delivery. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Dextran Hydrodynamic Polysaccharides-based nanoparticles as drug delivery systems. In addition, we evaluated the regeneration of SC after microporation. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Huggins, Maurice L. (1942). Biomaterials, 22, 1891-1898. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Structural and biocompatibility properties of dextran from For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Epub 2022 Feb 2. GPC Symp. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. The basic setup of a DLS instrument is shown in Figure 1. Journal of Controlled Release, 119, 5-24. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Increased TEWL values are evidence of micropores formation. European Polymer Journal, 43, 1744-1753. Ubbelohde, Leo. Doke SK, Dhawale SC. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Thermo Fisher Scientific. Dilute solution properties and degree of chain branching for dextran. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. 2009;8(2):1739. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Monolayers and Thin Films of Dextran Hydrophobically Modified. 1984;17(6):11703. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. 43-1, 115-147. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. (2013). Differences were considered statistically significant at p <0.05. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Webof overall macromolecular geometry and hydrodynamic draining. Polysaccharide hydrogels for modified release formulations. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). A solvent at this temperature is called a theta solvent [53]. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. 7 8 The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. The Mark-Houwink. Biological skin substitutes for wound cover and closure. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Nanoparticles in magnetic resonance imaging: from simple to dual In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. J Cosmet Laser Ther. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. (1954). Harding, Stephen E. (1997). Pharmaceut Sci Tech. Nanoparticles in magnetic resonance imaging: from simple to dual RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. (2003). The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Beer, M.U. Regulating dynamic signaling between hematopoietic stem cells dextran hydrodynamic radius. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. The authors declare that they have no competing interests. CAS Expert Opin Drug Deliv. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. hydrodynamic Nair SS. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Probiotics Antimicrob Proteins. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. With FITC-dextran 20 K, cumulative Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection eCollection 2018 Feb. Molecules. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Fluorescent and Biotinylated DextransSection 14.5 dextran 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. The Intrinsic Viscosity of Biological Macromolecules. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Indo AM J Pharm. 2008;26(11):12618. (2003). Bohdanecky, Miloslav (1996). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Morris, G.A. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. By using this website, you agree to our Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. Carbohydrate Polymers, 68, 477-488. Critical parameters for gel permeation Progr. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Carbohydrate Polymers 95 360-365. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). An undergraduate physical chemistry experiment. (1999). 20K. * shows a significant difference between the M.W. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Solution properties of branched dextran. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. 8580 Macromolecules 34, Notes - NIST In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). dextran hydrodynamic radius Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. The human skins were from the back or thigh of a 24-year-old woman. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Biomacromolecules 8, 2464-2475. Hennink, W.E. Structure Properties of Dextran. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Intrinsic viscosity of bovine serum albumin conformers. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The term f/f0 is sometimes denoted as P, Perrin constant. TEWL measurements were taken 30min after microporation of the human and pig skin. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. The mechanism of the dextran-induced red blood cell Aggregation. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Materiaux 1-5. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Appl Biochem Biotechnol. Porcine wound models for skin substitution and burn treatment. H&E stained images were analyzed using Image J software. Catiker, E.; Guner, A. principles of dynamic light scattering Molecular weight determination of polysaccharides. Pharm Res. ; Rani, K.Y. Figure4 shows the histological analysis of micropores in human cadaver skin. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Journal of Chemical Education, 80(9), 1036-1038. (2008). Carbohydrate Polymers, 79, 380-390. Banga AK. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Charge: Anionic. Progress in Polymer Science, 30, 38-70. Kasaai, Mohammad R. (2012). Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Drug Deliv. These studies were carried out at the Hisko (Gunpo, Korea). Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. The effect of branching is best recognized from the molecular weight dependencies of []. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. and transmitted securely. AATEX. ; Ghosh, T.K. The above data are not useful because it can not be compared with those obtained in this work. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Molecular weight (M v) and hydrodynamic properties can be calculated from there. dextran hydrodynamic radius. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Front Microbiol. Ther. Viscotek GmbH, Europe. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Carbohydrate Polymers, 78, 902-907. sharing sensitive information, make sure youre on a federal The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. In addition, the permeation rate was higher in pig skin than in human skin. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity.

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